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. 2019 Apr 26;2(3):e201900295. doi: 10.26508/lsa.201900295

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© 2019 Stevens et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S1. — (A, C, E) E2–Ub^Atto discharge assays with increasing concentrations of class VI RING domains. Assays were carried out with 4 μM of either TRIM25 or TRIM32 as a positive control, and up to 100 μM of either TRIM28 RING, TRIM24 RING, or TRIM33 RING as indicated. Reactions were monitored over 15 min. (B, D, F) Quantification of discharge assays: the loss of E2–Ub^Atto is plotted as the average of experimental duplicates.

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