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. 2019 Apr 26;2(3):e201900295. doi: 10.26508/lsa.201900295

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© 2019 Stevens et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S4. — Upper panel: full-length TRIM28 was incubated with E1, ubiquitin, ubiquitin^Atto, and a range of E2 enzymes as indicated. The reactions were monitored for auto-ubiquitination over 60 min. The TRIM25 RBCC was included in conjunction with UBE2D as a positive control. Lower panel: the results of the same assay carried out in the absence of any E3 ligase. The band labelled by an asterisk is likely an auto-ubiquitinated form of UBE2K.