Skip to main content
. 2019 Apr 18;13(5):1249–1267. doi: 10.1002/1878-0261.12483

Figure 6.

Figure 6

Levels of ZNF611, ZNF304, RIPK1, TNFRSF21, DUSP8 and HRAS are differentially regulated by miR‐122 in parental and radioresistant breast cancer cells. (A) Schematic illustration of the potential miR‐122 binding site in the 3′‐UTR of ZNF611, ZNF304, RIPK1 and DUSP8 genes. Letters labeled in blue indicate the seed region. Levels of ZNF611, ZNF304, RIPK1, TNFRSF21, DUSP8 and HRAS proteins were evaluated by western blot assays in (B) MCF‐7RR transfected with antagomiR‐122. (C) Parental MCF‐7 transfected with mimic‐miR122. (D) MDA‐MB‐231RR transfected with antagomiR‐122. (E) Parental MDA‐MB‐231 transfected with mimic‐miR122. β‐actin was used as an internal control. Images are representative of three independent experiments. Kaplan–Meier curves of RFS. Survival curves of high vs low expression of ZNF611, ZNF304, RIPK1, TNFRSF21, DUSP8 and HRAS of patients with (F) luminal breast cancer and (G) TNBC treated with radiotherapy. High or low gene expression levels according to > median or ≤ median expression levels each gene. Curves were compared using a log‐rank test *P ≤ 0.05.