Table 1.
Round of selection | FGFR1 (μg·mL−1) | Concentration of Tween‐20 (v/v) | Elution | Input phage (PFU) | Output phage (PFU) | Recovery (%) |
---|---|---|---|---|---|---|
1 | 100 | 0.1 | 0.2 m glycine pH 2.2 | 2 × 1011 | 1 × 107 | 5 × 10−3 |
2 | 70 | 0.3 | 0.2 m glycine pH 2.2 | 2 × 1011 | 12 × 107 | 6 × 10−2 |
3 | 50 | 0.5 | 0.2 m glycine pH 2.2/FGF1 | 2 × 1011 | 14 × 105/8 × 104 | 7 × 10−4/4 × 10−5 |