Skip to main content
. 2019 Mar 5;13(5):1121–1136. doi: 10.1002/1878-0261.12471

Figure 2.

Figure 2

HORAS5 is a stable cytoplasmic lncRNA in AR‐positive PCa cells. (A) Basal expression (qPCR) of HORAS5 in a panel of PCa cell lines. Gene expression was normalized to average of GAPDH and HPRT1 and shown relative to BPH1. (B) Taqman qPCR expression of HORAS5 long and short variants (as identified in the Ensembl database) in two CRPC‐derived cell lines, namely LNCaP and C4‐2. (C) qPCR expression of HORAS5 in the nuclear and cytoplasmic subcellular fractions of AR‐positive LNCaP cells. GAPDH and snoRNA55 are used as controls for cytoplasmic and nuclear fractions, respectively. Results from two independent experiments are plotted. (D) qPCR‐based transcript expression of HORAS5 and c‐Myc in LNCaP cells treated with alpha‐amanitin for various time‐points (0, 2, 5, 6, 10 h post‐treatment). Relatively stable GAPDH expression was used for normalization, and data are plotted relative expression at 0 h. Results from two independent experiments are plotted as means ± SD.