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Prevention of VGF induction in PC12 cells using siRNA silencing. Western blot confirming the maintenance of baseline levels of VGF upon conditioned medium (CnM) following siRNA (A). PC12 cell survival (using Annexin V/DAPI flow cytometry) (B), representative pictures of PC12 cell neurite extension (C), and quantification of the neurite extension (D) following VGF siRNA or nontargeting siRNA treatment. Scale bar = 50μm; 6B/D, ANOVA, *P < 0.05; error bars indicate SEM; n = 3.
