Skip to main content
. 2019 Mar 9;13(5):1002–1017. doi: 10.1002/1878-0261.12439

Figure 3.

Figure 3

Sarcosine treatment has stimulatory effects on the methyl‐donor SAMe and global methylation status of prostate cells. (A) Values of SAMe and SAH and their ratios (CMP, cellular methylation potential) in PNT1A, 22Rv1 and LNCaP cells nontreated and treated with sarcosine (1 μm). (B) Schematic depiction of roles of SAMe and SAH in C5‐methylation of cytosine to 5‐methylcytosine and the consequent effects on gene transcription. TF, transcription factor; MBP, methyl‐CpG binding proteins. Global methylation indices of (C) prostate and (D) nonprostate cell lines treated with sarcosine (1 μm). Methylation indices were analysed using the MDQ1 Imprint Kit. The values are expressed as the median of three replicates (n = 3) obtained from five independent experiments (n = 5). Ctrl: cells analysed upon 24‐h incubation in medium without addition of sarcosine. The vertical bars indicate the standard deviation. *P < 0.05, **P < 0.01 (paired t‐test) compared with Ctrl – nontreated cells.