Table 2. Statistical results and expression level analyses of all tested candidate reference genes in different tissues on account of their threshold cycle point values (Ct) as furnished by BestKeeper.
| Factor | CYC | RPL28S | ACTB | TUBB | EF1a | Ubiq | 18S RNA |
|---|---|---|---|---|---|---|---|
| N | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| GM[Ct] | 28.94 | 27.76 | 21.98 | 23.05 | 19.73 | 31.07 | 22.58 |
| AM[Ct] | 28.96 | 27.77 | 22.16 | 23.06 | 19.75 | 31.09 | 22.65 |
| Min[Ct] | 27.88 | 26.88 | 18.75 | 21.74 | 19.1 | 29.96 | 20.08 |
| Max[Ct] | 30 | 28.92 | 26.91 | 24.4 | 21.35 | 33.09 | 25.18 |
| SD[ ±Ct] | 0.89 | 0.48 | 2.67 | 0.77 | 0.66 | 0.94 | 1.72 |
| CV[%Ct] | 3.09 | 1.74 | 12.05 | 3.33 | 3.33 | 3.01 | 7.58 |
| Min[x-fold] | −2.09 | −1.84 | −9.37 | −2.47 | −1.55 | −2.17 | −5.63 |
| Max[x-fold] | 2.09 | 2.23 | 30.66 | 2.56 | 3.08 | 4.06 | 6.08 |
| SD[± x-fold] | 1.86 | 1.4 | 6.37 | 1.7 | 1.58 | 1.91 | 3.29 |