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. 2019 Apr 26;7:e6834. doi: 10.7717/peerj.6834

Table 2. Statistical results and expression level analyses of all tested candidate reference genes in different tissues on account of their threshold cycle point values (Ct) as furnished by BestKeeper.

Factor CYC RPL28S ACTB TUBB EF1a Ubiq 18S RNA
N 6 6 6 6 6 6 6
GM[Ct] 28.94 27.76 21.98 23.05 19.73 31.07 22.58
AM[Ct] 28.96 27.77 22.16 23.06 19.75 31.09 22.65
Min[Ct] 27.88 26.88 18.75 21.74 19.1 29.96 20.08
Max[Ct] 30 28.92 26.91 24.4 21.35 33.09 25.18
SD[ ±Ct] 0.89 0.48 2.67 0.77 0.66 0.94 1.72
CV[%Ct] 3.09 1.74 12.05 3.33 3.33 3.01 7.58
Min[x-fold] −2.09 −1.84 −9.37 −2.47 −1.55 −2.17 −5.63
Max[x-fold] 2.09 2.23 30.66 2.56 3.08 4.06 6.08
SD[± x-fold] 1.86 1.4 6.37 1.7 1.58 1.91 3.29