Table 4. Statistical results and expression level analyses of all tested candidate reference genes under culturing environment in salinity of 5ppt based on their threshold cycle point values (Ct) as provided by BestKeeper.
| Factor | CYC | RPL28S | ACTB | TUBB | EF1a | Ubiq | 18S RNA |
|---|---|---|---|---|---|---|---|
| N | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
| GM[Ct] | 33.15 | 30.59 | 23.35 | 27.22 | 28.48 | 35.1 | 32.13 |
| AM[Ct] | 33.26 | 30.63 | 23.41 | 27.29 | 28.52 | 35.18 | 32.14 |
| Min[Ct] | 28.87 | 29.51 | 21.44 | 25.37 | 26.78 | 30.01 | 31.06 |
| Max[Ct] | 38.67 | 33.94 | 27.08 | 32.12 | 32 | 37.79 | 34.17 |
| SD[±Ct] | 2.03 | 1.35 | 1.48 | 1.58 | 1.37 | 1.79 | 0.68 |
| CV[%Ct] | 6.1 | 4.4 | 6.31 | 5.8 | 4.79 | 5.09 | 2.11 |
| Min[x-fold] | −19.39 | −2.11 | −3.75 | −3.6 | −3.25 | −34.04 | −2.09 |
| Max[x-fold] | 46 | 10.21 | 13.26 | 29.88 | 11.54 | 6.45 | 4.12 |
| SD[± x-fold] | 4.08 | 2.55 | 2.78 | 3 | 2.58 | 3.46 | 1.6 |