Table 6. Statistical results and expression level analyses of all tested candidate reference genes under culturing environment in salinity of 25ppt on account of their threshold cycle point values (Ct) as furnished by BestKeeper.

Factor	CYC	RPL28S	ACTB	TUBB	EF1a	Ubiq	18S RNA
N	7	7	7	7	7	7	7
GM[Ct]	22.22	27.75	19.44	20.35	18.48	31.5	20.77
AM[Ct]	22.23	27.75	19.44	20.37	18.49	31.5	20.86
Min[Ct]	20.76	26.82	19.14	19.09	17.5	31.1	19.32
Max[Ct]	23.4	28.62	20.6	21.86	19.85	31.9	25.25
SD[±Ct]	0.64	0.58	0.33	0.87	0.52	0.22	1.57
CV[%Ct]	2.88	2.07	1.72	4.27	2.8	0.71	7.54
Min[x-fold]	−2.74	−1.9	−1.23	−2.39	−1.97	−1.31	−2.72
Max[x-fold]	2.26	1.83	2.23	2.85	2.59	1.32	22.39
SD[± x-fold]	1.56	1.49	1.26	1.83	1.43	1.17	2.97