Table 2.

Flow cytometry panel for ILCs

Antigen	Fluorochrome	Company	Clone
ILC1, 2, 3 with transcription factors
Lineage	AF700	Biolegend
CD45	PerCP‐Cy5.5	Biolegend	30‐F11
CD90.2	APC‐Cy7	Biolegend	30‐H12
IL‐7Rα	BV605	Biolegend	A7R34
Rorγt	PE	Biolegend	Q31‐378
GATA3	PE‐Cy7	BD	L50‐823
T‐bet	BV421	BD	04‐46
CD4	FITC	Biolegend	GK1.5

ILC1: Lin^‐CD90.2⁺CD45⁺IL‐7Rα⁺T‐bet⁺; ILC2: Lin^‐CD90.2⁺CD45⁺IL‐7Rα⁺GATA3⁺T‐bet^‐Rorγt^‐CD4⁻; ILC3: Lin^‐CD90.2⁺CD45⁺IL‐7Rα⁺Rorγt⁺CD4^+/−