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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2019 Mar 30;531:260–268. doi: 10.1016/j.virol.2019.03.018

Fig. 5. SAMHD1 does not interact with MxB.

Fig. 5

293T cells were co-transfected with SAMHD1-FLAG and either MxB-FLAG or empty vector LPCX. Cells were lysed 24 h post-transfection and analyzed by Western blot using anti-HA and anti-FLAG antibodies (Input). Subsequently, lysates were immunoprecipitated by using anti-FLAG agarose beads, as described in Methods. Anti-FLAG agarose beads were eluted using the 3X FLAG peptide. Elutions were analyzed by Western blot using anti-HA and anti-FLAG antibodies (Immunoprecipitation). WB, Western blot; IP, Immunoprecipitation.