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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2019 Mar 30;531:260–268. doi: 10.1016/j.virol.2019.03.018

Fig. 6. Contribution of MxB capsid-binding ability to SAMHD1 restriction of HIV-1.

Fig. 6

The ability of endogenous SAMHD1 to bind to in vitro assembled HIV-1 CA-NC complexes were measured in extracts from cells that do not express MxB (THP-1-MxB-KO) as described in Methods. As a control, similar experiments were performed using extracts from control THP-1 cells that do express MxB. Input and Pellets fractions were analyzed by Western blot using anti-MxB, anti-SAMHD1, and anti-p24 antibodies. Similar results were obtained in three independent experiments and a representative experiment is shown. * denotes P value < 0.05, ** denotes P value < 0.005, *** denotes P value < 0.0001 as determined by One-way ANOVA Tukey’s multiple comparisons test.