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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2019 Mar 30;531:260–268. doi: 10.1016/j.virol.2019.03.018

Fig. 9. Phosphorylation state of SAMHD1 at position T592 in the absence of MxB.

Fig. 9

Lysates from PMA-treated THP-1 and THP-1-MxB-KO cells were analyzed by Western blot analysis using anti-MxB, anti-SAMHD1, and anti-phospho-T592-SAMHD1. As loading control, samples were analyzed for the expression of GAPDH. As a positive control for phosphorylation, THP-1 cells that were not treated with PMA were utilized. Experiments were done in triplicate and a representative experiment is shown.