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. 2019 Apr 26;14:2945–2959. doi: 10.2147/IJN.S199781

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© 2019 Liu et al. This work is published and licensed by Dove Medical Press Limited

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Effect of EO-AuNPs on LPS-induced inflammatory mediators production in RAW 264.7 cells. (A) Cell viability was measured by MTT assay. (B) The concentration of NO in medium was measured by Griess method. (C–E) The production of TNF-α, IL-1β, and IL-6 in medium was measured using ELISA kits. Data are representative of three independent experiments and values are shown as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 compared to non-treated control. ^#P<0.05, ^##P<0.01, ^###P<0.001 compared to the LPS control.

Abbreviations: TNF-α, tumor necrosis factor-α; EO-AuNPs, Euphrasia officinalis-gold nanoparticles; LPS, lipopolysaccharide; NO, nitric oxide; SEM, standard error of the mean.