Table 2.
Combined antibodies | Primary antibody source | Mono‐ or poly‐clonal antibodies | Host/dilution | 2nd antibody host/source/dilution |
---|---|---|---|---|
eGFP/mSCFR | Abcam, Cambridge, MA, USA | Poly‐/poly‐ | Chicken/goat | Rabbit/donkey |
mSCFR/TMEM16A | R&D Systems Inc., Minneapolis, MN, USA R&D Systems Inc., Minneapolis, MN, USA/Abcam, Cambridge, MA, USA |
Poly‐/poly‐ |
1:1000/1:100 Goat/ rabbit 1:100/1:800 |
Jackson IR West Grove PA, USA/Life Technologies Eugene, OR, USA 1:800/1:1000 Donkey/donkey Life Technologies, Eugene, OR, USA for both 1:1000/1:1000 |
Note: mSCFR: anti‐mouse stem cell factor/Kit receptor; TMEM16A: anti‐anoctamin 1 (Ano1).