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. 2019 Mar 28;597(9):2337–2360. doi: 10.1113/JP277335

Table 2.

Details of antibody combinations used for double‐label immunohistochemistry

Combined antibodies Primary antibody source Mono‐ or poly‐clonal antibodies Host/dilution 2nd antibody host/source/dilution
eGFP/mSCFR Abcam, Cambridge, MA, USA Poly‐/poly‐ Chicken/goat Rabbit/donkey
mSCFR/TMEM16A R&D Systems Inc., Minneapolis, MN, USA
R&D Systems Inc., Minneapolis, MN, USA/Abcam, Cambridge, MA, USA
Poly‐/poly‐ 1:1000/1:100
Goat/ rabbit
1:100/1:800
Jackson IR West Grove PA, USA/Life Technologies Eugene, OR, USA
1:800/1:1000
Donkey/donkey
Life Technologies, Eugene,
OR, USA for both
1:1000/1:1000

Note: mSCFR: anti‐mouse stem cell factor/Kit receptor; TMEM16A: anti‐anoctamin 1 (Ano1).