Table 2.
Relative abundance of microbial phyla and genera
| Abundance (%) | ||||
|---|---|---|---|---|
| Young | Older | W score | Centred log ratio mean difference F statistic | |
| Phyla altered by ageing | ||||
| Proteobacteria | 0.02 (0.01, 0.05) | 0.78 (0.38, 1.58) | 9* | 2.569 |
| Verrucomicrobia | 0.01 (0.01, 0.01) | 0.22 (0.13, 0.97) | 9* | 2.377 |
| TM7 | Not detected | 0.08 (0.03, 0.12) | 10* | 0.971 |
| Phyla not significantly altered by ageing | ||||
| Bacteroidetes | 42.3 (32.8, 56.7) | 49.1 (43.1, 57.8) | 3 | –0.663 |
| Firmicutes | 46.5 (41.3, 53.2) | 40.8 (33.1, 54.8) | 3 | –0.884 |
| Tenericutes | 0.75 (0.50, 1.34) | 1.17 (0.75, 2.47) | 3 | –0.423 |
| Deferribacteres | 0.31 (0.11, 0.78) | 0.11 (0.04, 0.38) | 3 | –1.602 |
| Actinobacteria | 0.15 (0.10, 0.20) | 0.25 (0.12, 0.38) | 3 | –0.160 |
| Cyanobacteria | Not detected | 0.01 (0.01, 0.01) | 4 | –0.185 |
| Fusobacteria | Not detected | 0.01 (0.01, 0.01) | 3 | –0.704 |
| Unknown | 0.78 (0.60, 0.96) | 0.41 (0.28, 0.83) | 5 | –1.298 |
| Genera altered by ageing | ||||
| p_Bacteroidetes; g_Bacteroides | 0.01 (0.01, 0.01) | 3.16 (2.38, 4.88) | 164* | 5.413 |
| p_Verrucomicrobia; g_Akkermansia | 0.01 (0.01, 0.01) | 0.22 (0.13, 0.97) | 159* | 3.030 |
| p_Proteobacteria; g_Desulfovibrio | 0.01 (0.01, 0.01) | 0.35 (0.15, 1.16) | 155* | 2.734 |
| p_Firmicutes; g_Turicibacter | 3.99 (0.78, 5.83) | 0.01 (0.01, 0.05) | 163* | –4.352 |
| p_Firmicutues; f_Clostridiaceae; g_ | 3.21 (0.01, 5.86) | 0.01 (0.01, 0.01) | 160* | –3.328 |
| p_Bacteroidetes; g_Parabacteroides | 4.16 (1.81, 8.33) | 0.24 (0.16, 0.57) | 160* | –2.727 |
| p_Firmicutes; _; _; _; _ | 1.80 (0.05, 9.17) | 0.06 (0.03, 0.09) | 153* | –2.508 |
| Genera with a non‐significant trend in difference between young and old mice | ||||
| p_TM7; f_F16; g_ | Not detected | 0.08 (0.03, 0.12) | 137 | 1.623 |
| p_Bacteroidetes; f_Rikenellaceae; g_ | 0.39 (0.01, 3.04) | 2.52 (0.96, 3.53) | 131 | 1.958 |
| p_Proteobacteria; g_Bilophila | Not detected | 0.06 (0.01, 0.14) | 119 | 1.458 |
| p_Firmicutes; g_Lactobacillus | 2.39 (1.22, 4.67) | 8.34 (4.21, 11.40) | 97 | 1.139 |
Median relative abundance (% of total microbes) and analysis W scores determined using ANCOM (old vs. young) of all major microbial phyla and microbial genera with differential abundance between young and old mice. Abundance data are the 50th (25th, 75th) percentiles. The W score indicates the number of statistical subhypotheses that have passed for a given taxon. *Statistical significance. ‘p’, phylum; ‘f’, family; ‘g’, genus. ‘g__’ and the next highest known order are given for operational taxonomic units that do not have a specific genus name.