Figure 10. Hyperpolarization of NTS neurons incubated in high glucose (10 mM) does not rescue the response to a low glucose challenge and tolbutamide application.

A, neurons incubated in high glucose that had more positive resting membrane potentials (RMP) were hyperpolarized and challenged to a low glucose solution and tolbutamide application, respectively. B, comparison of the membrane potential change (ΔV _m) induced by low glucose between high glucose‐incubated responsive neurons (R) and the hyperpolarized neurons (Hyperpol) shown in A. C, comparison of the ΔV _m induced by tolbutamide between neurons pre‐incubated in 5 mM and 10 mM glucose shown in A. Tolb, tolbutamide. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001 [Color figure can be viewed at wileyonlinelibrary.com]