Table 5.

Locomotion assays

	Flight index		WBF (Hz)		Jump ability (cm)
	15°C	20°C	15°C	20°C	15°C	20°C
Control	2.29 ± 0.12	4.14 ± 0.16	155 ± 1.81	197 ± 2.08	1.70 ± 0.10	1.84 ± 0.09
R249Q/R249Q	1.30 ± 0.11*	1.78 ± 0.11*	126 ± 3.78*	158 ± 1.82*	1.14 ± 0.08*	1.44 ± 0.09*
R249Q/+	1.53 ± 0.11*	3.50 ± 0.17* , +	146 ± 2.33* , +	188 ± 4.07* , +	1.60 ± 0.06+	1.89 ± 0.09+

^*Significant difference from the control; ⁺significant difference between homozygous and heterozygous lines (one‐way ANOVA; P < 0.05). n = 111 for free flight, n = 10 for wing beat frequency (WBF), and n = 10 for jump tests, except for homozygous R249Q flies’ WBF at 15°C (n = 3) and at 20°C (n = 5) because homozygous flies had difficulty beating their wings for 20 consecutive seconds, which often caused unreliable WBF measurement.