Table 4.

Pearson's correlation matrix for 15 analysed MiSeq run metrics. Strong correlations (values between 0.80–1.00) are indicated in green; moderate correlations (0.50–0.79) are in yellow, and values with no color indicate weak correlations (0.00–0.49)

	% Clusters PF	%≥Q30 (Overall)	%≥Q30 (R1)	%≥Q30 (IR1)	%≥Q30 (IR2)	%≥Q30 (R2)	Phasing (R1)	Prephasing (R1)	Phasing (R2)	Prephasing (R2)	Total reads	Reads PF	Total yield	Q30 yield	Cluster density
% Clusters PF	*
%≥Q30 (Overall)	0.54	*
%≥Q30 (R1)	0.49	0.89	*
%≥Q30 (IR1)	0.52	0.46	0.38	*
%≥Q30 (IR2)	0.55	0.5	0.41	0.6	*
%≥Q30 (R2)	0.52	0.9	0.77	0.46	0.5	*
Phasing (R1)	−0.3	−0.32	−0.38	−0.29	−0.3	−0.27	*
Prephasing (R1)	−0.14	−0.58	−0.67	−0.16	−0.15	−0.48	0.26	*
Phasing (R2)	−0.03	0.05	0.03	0.09	−0.19	0.04	0.13	−0.03	*
Prephasing (R2)	−0.02	−0.17	−0.2	0.02	−0.12	−0.15	0.11	0.31	0.56	*
Total reads	−0.14	−0.15	−0.13	0.04	0.13	−0.15	−0.04	−0.05	−0.29	−0.23	*
Reads PF	0.16	0.02	0.02	0.23	0.32	0.02	−0.16	−0.1	−0.29	−0.23	0.94	*
Total yield	0.14	−0.01	0.01	0.21	0.3	−0.01	−0.16	−0.09	−0.3	−0.24	0.93	0.99	*
Q30 yield	0.23	0.2	0.19	0.3	0.4	0.2	−0.22	−0.18	−0.28	−0.34	0.86	0.96	0.96	*
Cluster density	−0.25	−0.2	−0.16	−0.03	0.05	−0.21	−0.04	−0.08	−0.27	−0.21	0.9	0.82	0.84	0.77	*