Table 2.
Primary antibodies and immunocytochemical techniques
| # | Antibody | Origin | Target | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|---|---|
| 1 | CD3 | Rabbit (mAB) | T‐cells | 1:2000 | St (E) | RM‐9107‐S; Neomarkers |
| 2 | Iba‐1 | Rabbit (pAB) | Ionized calcium binding adaptor molecule 1 | 1:3000 | St (E) | 019‐19741; Wako |
| 3 | CD68 | Mouse (mAB; IgG1) | CD68 110‐kD transmembrane glycoprotein in macrophages | 1:100 | St (E) | M0814; Dako, |
| 4 | HLA‐DR | Mouse (mAB; IgG1) | MHC Class II antigen | 1:100 | St (C) | M0775; Dako, |
| 5 | p22phox | Rabbit (pAB) | NADPH oxidase protein | 1:100 | St (C) | sc‐20781; Santa Cruz |
| 6 | TMEM119 | Rabbit (pAB) | Transmembrane protein 119 | 1:100 | ‐ | HPA051870; Sigma‐Aldrich |
| 7 | P2RY12 | Rabbit (pAB) | Purinergic receptor | 1:2500 | St (E) | Harvard, Dr. Butovsky |
| 8 | HC10 | Mouse (mAB; IgG2a) | Heavy chain of MHC Class I | 1:2000 | St (E) | Stam et al., 1990 |
| 9 | Ferritin | Rabbit (pAB) | Iron storage protein | 1:1000 | St (E) | MO (F5012); Sigma‐Aldrich |
| 10 | CD206 | Mouse (mAB; IgG1) | Mannose receptor | 1:100 | St (E) | ab117644; abcam |
| 11 | CD163 | Mouse (mAB; IgG1) | Haemoglobin‐haptoglobin scavenger receptor | 1:1000 | St (C) | NCL‐CD163; Novocastra |
| 12 | iNOS | Rabbit (pAB) | Inducible nitric oxide synthase I | 1:200 | St (E) | PA1‐37925; Thermo Scientific |
| 13 | CD86 | Goat (pAB) | Costimulatory T‐cell signal | 1:250 | St (C) | AF‐141‐NA; R&D Systems |
| 14 | GFAP | Rabbit (pAB) | Glial fibrillary acidic protein | 1:3000 | St(E) | Z0334; Dako |
| 15 | TG2 | Mouse (mAB) | Transglutaminase 2 | 1:5000 | St (E) | AB‐3; NeoMarkers |
MAB, monoclonal antibody; pAB, polyclonal antibody; ST (E), antigen retrieval by steaming in EDTA buffer (pH: 9); St(C), antigen retrieval by steaming in citrate buffer (pH: 5).