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. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: Exp Eye Res. 2018 Apr 25;173:64–72. doi: 10.1016/j.exer.2018.04.015

Fig. 4 — Cultured goblet cells were preincubated with the PLC inhibitor U73122 and its negative control U73343, both at 10⁻⁶ M, for 15 min (A and B), 2-APB (10⁻⁶ and 10⁻⁵ M) for 10 min (C and D), thapsagargin for 15 min (E and F) or Ro 31–8220 for 10 min (G and H). [Ca²⁺]_i over time is shown in A, C, E, and G. Change in peak [Ca²⁺]_i is shown in B, D, F, and H. Data are mean ± SEM from 3 (A and B), 7 (C and D), 6 (E and F) and 6 (G and H) independent experiments. * indicates significant difference from basal; # indicates significance from RvE1 alone.

Fig. 4 — Cultured goblet cells were preincubated with the PLC inhibitor U73122 and its negative control U73343, both at 10⁻⁶ M, for 15 min (A and B), 2-APB (10⁻⁶ and 10⁻⁵ M) for 10 min (C and D), thapsagargin for 15 min (E and F) or Ro 31–8220 for 10 min (G and H). [Ca²⁺]_i over time is shown in A, C, E, and G. Change in peak [Ca²⁺]_i is shown in B, D, F, and H. Data are mean ± SEM from 3 (A and B), 7 (C and D), 6 (E and F) and 6 (G and H) independent experiments. * indicates significant difference from basal; # indicates significance from RvE1 alone.