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. 2019 Apr 17;13(4):e0007343. doi: 10.1371/journal.pntd.0007343

Fig 2. Fetal outcomes after maternal infection with ZIKV strains.

Fig 2

(a) Rate of grossly normal (black) versus abnormal (red) fetuses at E14.5 after maternal infection at E7.5. An abnormal fetus was defined as resorption-prone. Data presented are for individual fetuses from 5–6 litters per treatment group. The n for each group is indicated above each bar. ****p<0.0001; ns, not significant (Fisher’s exact test). (b) Fetus size as assessed by crown-rump length (CRL) in mm using ImageJ software. CRL was only measured for fetuses determined to be grossly normal at E14.5. ****p<0.0001; ns, not significant (unpaired Student’s t-test). (c) Representative images of fetuses on E14.5 from each treatment group. Scale bar, 2 mm. PBS characterized as normal. ZIKV-PR-IC, ZIKV-PR-N139S, ZIKV-DAK characterized as abnormal. (d-f) Viral burdens were measured by qRT-PCR assay from individual homogenized placentas (d), fetuses (e), and concepti (when the fetus and placenta could not be separated due to severe resorption). (f) Symbols represent individual placenta, fetus, or conceptus from 3–5 independent experiments for each treatment group. Bars represent the mean viral burden of each treatment group. *p<0.05; ns, not significant (one-way ANOVA).