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. 2019 Mar 18;8(4):1721–1730. doi: 10.1002/cam4.2040

Figure 6.

Figure 6

miR‐181a‐5p regulated EMT, apoptosis and senescence via targeting TGFβI. (A) Predict binding sites between TGFβI and miR‐181a‐5p. (B) TGFβI expression was detected in human normal cervical cell line Etc1/E6E7 and in cervical cancer lines HeLa, C4‐1, SiHa and Ca Ski cells by qRT‐PCR. GAPDH was used as the internal control. *P < 0.05,**P < 0.01, ***P < 0.001 compared with Etc1/E6E7 group. (C) SB431542 was introduced to inhibit TGFβI. HeLa cells were transfected with Ad‐CDKN2B‐AS1 with or without SB431542. The relative protein levels of TGFβI, VEGFA, and Vimentin were detected in HeLa cells by western blot. GAPDH was used as internal control. (D) The cell invasion abilities were quantified. (E) Apoptosis rate was quantified. (F) Senescence was detected by Senescence β‐Galactosidase Staining Kit. **P < 0.01, compared with Ctrl group. ##P < 0.01, compared with SB431542 group.