Table 1.

Sequences of the primer pairs used to analyze the genes

ID	Primer sequence (5′‐3′)	Tm (°C)	Ct
hsa_circ_0005505	R: GCCAGCTGCTTGAAAGTCTC F: TGCAGTGTAAGAAGCATTGGA	53	25‐32
hsa_circ_0001394	R: TCCATCAGTCATCTTGGTCCA F: TTCATGAAGCTGAGGAGGGG	55	25‐27
hsa_circ_0000798	R: GTACCTGCATCTGGGGTGAC F: ACTCCTGGACAAGGATCTGC	55	23‐25
hsa_circ_0004771	R: AGCTCACAATCCAAACACTTCC F: ACTTTTCAACAGCCTTCTCAAT	48	28‐32
hsa_circ_0001074	R: TTACTCTTTGATTTACGACTGC F: GTAACTCTGTCCTTATTATCGG	46	31‐35
hsa_circ_0067735	R: TCTCTGCACTCTTCACATTCCA F: TGACTTGTGCCTATTATTCTGC	48	26‐32
GAPDH	R: GGTCTGGGAGCCTGGAAAA F: TTCGCTCCTGGAAGATGGTAAT	46‐55	16‐24

Ct, cycle threshold; F, forward; R, reverse; Tm, annealing temperature.