Table 1.
Composition and physical characterization (size, PDI, zeta potential, and LC) of the four AMX-loaded LNPs and the corresponding unloaded LNPs
| Formulation | P1 | F1 | P2 | F2 | P3 | F3 | P4 | F4 |
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| mg | ||||||||
| Cetyl palmitate | 178.5 | 178.5 | 178.5 | 178.5 | 178.5 | 178.5 | 178.5 | 178.5 |
| Tween 80 | 130 | 130 | 125 | 125 | 110 | 110 | 105 | 105 |
| Linolenic acid | – | – | 5 | 5 | – | – | 5 | 5 |
| DOPE | – | – | – | – | 20 | 20 | 20 | 20 |
| AMX | – | 19.9 | – | 19.9 | – | 19.9 | – | 19.9 |
|
| ||||||||
| Size (nm) | 291±5 | 284±11 | 243±17 | 240±16 | 237±12 | 236±18 | 178±9 | 197±2 |
| PDI (±0.02) | 0.14 | 0.14 | 0.12 | 0.12 | 0.12 | 0.14 | 0.11 | 0.12 |
| ζ (mV) | -48±1 | -40±1 | -39±5 | -46±4 | -42±4 | -37±4 | -42±2 | -43±3 |
| LC (%) | – | 6.3±0.1 | – | 6.8±0.4 | – | 5.7±0.6 | – | 7.5±0.2 |
Abbreviations: AMX, amoxicillin; DOPE, dioleoylphosphatidylethanolamine; LC, loading capacity; LNPs, lipid nanoparticles; PDI, polydispersity.