Comparison of levels of apoptosis in decidual tissues among the control, abortion and RM groups. (A) Apoptosis was determined by terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labelling in decidual tissues from women in the normal pregnancy (n=34), abortion (n=26) and RM (n=21) groups, respectively. Distributions of (B) Bax and (C) Bcl-2 in decidual tissues were detected by immunohistochemistry (magnification, ×400). (D) Protein levels of Bax and Bcl-2 in decidual tissues were measured by western blotting. (E) The expression of Bax on trophoblasts was analyzed by dual immunofluorescence (magnification, ×400). (F) The expression of Bcl-2 on trophoblasts was analyzed by dual immunofluorescence (magnification, ×400). Scale bars, 100 µm. *P<0.05 vs. the control group. RM, recurrent miscarriage; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; DAPI, 4′,6-diamidino-2-phenylindole.