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. 2019 Apr 4;43(6):2387–2397. doi: 10.3892/ijmm.2019.4157

Figure 2.

Figure 2

Effect of miR-576-3p on hypoxic-treated glioma cells in vitro. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the relative expression levels of the transfected LV-miR-576-3p in the indicated cells (U87 and U251). Effect of LV-miR-576-3p or LV-miR-NC on the (B) migration of U87 and U251 cells subsequent to hypoxic incubation for 24 h were determined using a wound healing assay (scale bar, 200 µm), which was then (C) quantified. Effect of LV-miR-576-3p or LV-miR-NC on the proangiogenic abilities of glioma cells subsequent to hypoxic incubation for 24 h were determined using (D) a Transwell migration assay (scale bar, 100 µm) and (E) quantified, and (F) tube formation assays (scale bar, 300 µm) which were then (G) quantified. All experiments were repeated three times. **P<0.01 and ***P<0.001 with comparisons shown by lines. miR, microRNA; LV, lentivirus; NC, negative control.