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. 2019 Apr 4;43(6):2291–2302. doi: 10.3892/ijmm.2019.4156

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Copyright: © Pu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Construction of the IL-18 plasmid. (A) The coding sequence of IL-18 was amplified by polymerase chain reaction. The product was visualized by agarose gel electrophoresis. (B) Double digests showing the vector and the IL-18 insert. (C) Alignment of the cloned product, as verified by sequencing, to the IL-18 gene sequence. (D) BL21 bacteria expressing the IL-18 plasmid after kanamycin selection. (E) Confirmation of successful IL-18 protein expression by western blot analysis. IL, interleukin.