Figure 1.

NEF characterization by AFM and western blotting. (A) Morphological analysis by AFM of C and DS NEF preparations. Images are representative of 3 independent experiments. (B) NEF obtained from C and DS plasma and the corresponding supernatants (SN_C and SN_DS) were immunoblotted for the indicated extracellular vesicles and high density lipoprotoeins markers (see text for details). Due to antibody-unspecific signals, the original image of western blotting membranes has been cropped. The uncropped, original image is available upon request. DS, Down syndrome; C, healthy control; NEF, nanoparticle-enriched fraction; AFM, atomic force microscopy; miR, microRNA; SNC, SN_DS, C and DS plasma corresponding supernatants; M, marker; CD, cluster of differentiation.