Fig 10. 2-Deoxyglucose (2-DG) uptake and phosphorylation of AMP-activated protein kinase (AMPK) on Thr¹⁷² (pAMPK Thr¹⁷²) in ex vivo incubated epitrochlearis from WT and AS160-KO rats.

Paired muscles were incubated without (open bars) or with (filled bars) 2 mM AICAR. Data were analyzed by two-way ANOVA, and Holm-Sidak post hoc analysis was used to identify the source of significant variance. (A) *P<0.01 for no AICAR versus AICAR within the same genotype; ^†P<0.01 for WT versus KO with the same AICAR concentration. Values are means ± SEM. N = 5–9 rats per group. (B) Bar graphs represent the ratio of the values for the immunoblots and their respective loading controls (Memcode stain). ^†P<0.01 (in WT) for no AICAR versus AICAR; ^‡P<0.005 for no AICAR versus AICAR in KO. Values are means ± SEM. N = 5–9 rats per group.