Table 2. Relative myosin heavy chain isoform composition of skeletal muscles.
%MHC-I | %MHC-IIA | %MHC-IIB | %MHC-IIX | |||||
---|---|---|---|---|---|---|---|---|
WT | KO | WT | KO | WT | KO | WT | KO | |
EDL | 0 | 0 | 8.9 ±1.1 | 10.1 ±1.0 | 55.0 ±0.6 | 52.9 ±4.4 | 36.1 ±0.5 | 37.0 ±3.6 |
EPI | 5.6 ±1.9 | 5.9 ±2.1 | 11.9 ±0.5 | 9.5 ±1.0 | 53.1 ±2.0 | 56.5 ±2.7 | 29.4 ±2.5 | 28.1 ±1.0 |
GAS | 6.7 ±3.4 | 7.2 ±2.3 | 11.1 ±3.6 | 12.6 ±4.2 | 43.9 ±3.8 | 41.2 ±6.0 | 38.2 ±3.3 | 39.1 ±7.7 |
SOL | 87.9 ±4.4 | 83.6 ±1.4 | 12.1 ±4.4 | 16.4 ±1.4 | 0 | 0 | 0 | 0 |
Values are means ±SEM (n = 3–6 rats per genotype). WT, wildtype. KO, AS160-KO. Myosin heavy chain (MHC) isoform is expressed as relative values (%) for each of the 4 skeletal muscles: extensor digitorum longus (EDL), epitrochlearis (EPI), gastrocnemius (GAS), and soleus (SOL).