Fig 6. Ca2+/calmodulin-dependent protein kinase II (CaMKII) activity and phosphorylation after doxorubicin (Dox) treatment with glucose regulated protein 78 (GRP78) overexpression.
Neonatal rat cardiomyocytes were transfected with adeno-associated virus 6 expressing GRP78 (AAV-GRP78) or Luciferase (AAV-Luc) two days prior to treatment with 1μM Dox. (A) Dox induced time dependent changes in (monomeric) phospholamban (PLN) levels (n = 4) and phosphorylation at Thr17 with AAV-GRP78 treatment (n = 7). (B) Time dependent changes in CaMKII levels (n = 4) and autophosphorylation at Thr286 after Dox treatment (n = 4). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene. Phosphorylated protein was normalized for total expression of respective protein *p < 0.05 for 2-way ANOVA with Bonferroni post hoc testing.