Figure 2.

Heterologous complementation of H. pluvialis phytoene synthase in E. coli. The HPLC analyses of E. coli carotenoids following the cotransformation of H. pluvialis phytoene synthase gene together with either pACCAR25ΔcrtB or plasmid pACCRT-E are shown in A and C, respectively. HPLC separations of carotenoid pigments extracted from E. coli cells carrying either the complementation plasmids pACCAR25ΔcrtB or pACCRT-E are shown in B and D. In addition, the absorption spectra of peaks 1 through 4 (zeaxanthin and zeaxanthin glycosides) as well as the spectrum of peak 5 (phytoene) are shown in E and F.