Video 4. DAG signaling is LOV2-dependent.

Download video file ^{(977.3KB, mp4)}

DOI: 10.7554/eLife.42498.014

Time course showing that in the absence of LOV2, the cells fail to exhibit blue light-induced changes in DAG accumulation. Jurkat cells expressing both the Zdk-CAR and DAG reporter were passively adhered to an SLB lacking LOV2 with a biotinylated anti-β2 microglobulin antibody and were exposed to the same pulses of blue light as in Video 1. We observed spontaneous flashes of DAG but no changes in DAG accumulation in response to blue light illumination. Cells were imaged in TIRF with a 561 nm laser. The mean response of the cells is plotted.