Video 6. Light-based titration of CAR occupancy.

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DOI: 10.7554/eLife.42498.016

Time course showing the quantification of receptor occupancy (via LOV2 localization) in response to intermediate blue-light intensities. Because the 488 nm laser strongly activates LOV2, LOV2 localization can only be imaged at the end of a pulse of blue light (every five minutes). Cells are the same as in Video 5, imaged in TIRF with a 488 nm laser. The mean response of the cells is plotted.