Table 1. Plasmids use in this study.
The plasmid name, the expressed protein, and a brief description of the construct are given. The entire coding regions of all constructs were verified by Sanger Sequencing. Plasmids and detailed maps are available from Addgene.
| Plasmid | Expressed protein | Backbone | Description |
|---|---|---|---|
| pDT326 | DAG reporter | pHR (James and Vale, 2012) | C1 domains mPKCθ (aa 45–166)-HaloTag |
| pDT481 | Zdk1 | pETM11-SUMO3 | 10xHis-TEV-SUMO3-KCK-SpyCatcher-GS linker-Zdk1 |
| pDT523 | ZAP70 reporter | pHR | hZAP70-mCherry |
| pDT537 | Zdk-CAR | pHR (James and Vale, 2012) | IgK ss-HA tag-Zdk1-GS linker-hCD8α (aa 22–208)-mCD3ζ (aa 52–164)-tagBFP |
| pDT552 | LOV2 | pETM11-SUMO3 | 10xHis-TEV-AviTag-KCK-LOV2 V529N |