Figure 3. Terfanadine (10 $\mu$M) abolished the increase in neuronal excitability normally observed following coincident ACh release.

(A) Control and Terf responses from the same neurons with identical light pulse trains timing in each condition. **p=0.00201, T = 5.884; n.s. p=0.254, T = 2.063. (B) Quantification of total number of APs across conditions. Total number of APs was increased upon ACh release (*p=0.0158, T = 4.719). This effect was blocked by application of terfenadine (n.s. p=1, T = 0.952). Application of Terfenadine significantly increased the total number of APs without light (step, Control vs. Terf: **p=0.00498, T = 6.141; all paired t-test, Bonferroni corrected). (C) plot of reduction in afterdepolarization response amplitude (indicated by red arrowhead in example Control step + light orange trace) by Terf. ** Control step vs step + light: p=0.00992, T = 5.257; n.s. Terf step vs step + light P=0.267, T = 2.107; * Control step + light vs Terf step + light: p=0.0142, T = 4.843; all paired t-tests, Bonferroni corrected.