| Antibodies |
|
|
| NK1.1 (PK136) – BV711 |
Biolegend |
108745; RRID:AB_2563286 |
| CD3ε (145-2C11) – PE/Cy5 |
Biolegend |
100309, RRID:AB_312674 |
| CD19 (6D5) – BV605 |
Biolegend |
115540, RRID:AB_2563067 |
| CD45.1 (A20) – Percp/Cy5.5 |
Biolegend |
110728, RRID:AB_893346) |
| CD45.2 (104) – BUV395 |
BD |
564616 |
| IFN-γ (XMG1.2) – PE |
Biolegend |
505808, RRID:AB_315402 |
| CD107a (1D4B) – A647 |
Biolegend |
121609, RRID:AB_571990 |
| NKG2D (CX5) – PE |
Biolegend |
130207, RRID:AB_1227713 |
| NKp46 (29A1.4) – FITC |
Biolegend |
137606, RRID:AB_2298210 |
| CD11b (M1/70) – PE/Cy7 |
Biolegend |
101216, RRID:AB_312799 |
| CD69 (H1.2F3) – PE/Dazzle594 |
Biolegend |
104536, RRID:AB_2565583 |
| 4-1BB (17B5) – PE |
Biolegend |
106106, RRID:AB_2287565 |
| STING (clone 41) |
Millipore |
MABF213 |
| Bacterial and Virus Strains |
|
|
| HSV1 |
ATCC |
ATCC VR-1487 (KOS) |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| 2’3’-cGAMP |
Produced in-house |
N/A |
| Vaccinia 70mer |
Invivogen |
tlrl-vav70n |
| ISD DNA |
Invivogen |
tlrl-isdn |
| Herring Testes HT-DNA |
Sigma-Aldrich |
D6898 |
| Poybrene |
Sigma-Aldrich |
TR-1003 |
| Cytofix/Cytoperm |
BD |
554722 |
| GolgiPlug |
BD |
555029 |
| GolgiStop |
BD |
554724 |
| CFSE |
ThermoFisher Scientific |
C34554 |
| Cytarabine ARA-C |
Cayman Chemical |
16069 |
| Critical Commercial Assays |
|
|
| QuikChange Site Mutagenesis kit |
Agilent technologies |
210514 |
| RNAeasy Mini kit |
Qiagen |
74104 |
| iScript reverse transcripase |
Bio-Rad |
1708841 |
| SSO-Fast Eva Green Supermix |
Bio-Rad |
1725203 |
| Experimental Models: Cell Lines |
|
|
| 293T |
ATCC |
CRL-3216 |
| B16-BL6 |
Laboratory of James P. Allison |
N/A |
| B16-BL6-Cgas−/−
|
This paper |
N/A |
| RMA-S |
Laboratory of Klas Karre |
N/A |
| RMA |
Laboratory of Klas Karre |
N/A |
| RMA-RAE-1ε |
Produced in house |
N/A |
| THP1 |
Invivogen |
thpd-nfis |
| THP1-TMEM173−/−
|
Invivogen |
thpd-kostg |
| MC38-GFP/Luc |
Laboratory of Dr. Michel Dupage |
N/A |
| Experimental Models: Organisms/Strains |
|
|
| C57BL/6J |
Jackson Laboratory |
000664 |
| C57BL/6J Tmem173−/−
|
Jackson Laboratory |
017537 |
| C57BL/6J Cgas−/−
|
This paper |
N/A |
| C57BL/6J Ifnar1−/−
|
Jackson Laboratory |
028228 |
| C57BL/6J Irf3−/−
|
Laboratory of Dr. T. Taniguchi |
N/A |
| C57BL/6J Ly5.1 |
Jackson Laboratory |
002014 |
| C57BL/6J B2m−/−
|
Jackson Laboratory |
002087 |
| C57BL/6J Rag2−/−
|
Jackson Laboratory |
008449 |
| C57BL/6J NKp46iCRE
|
Laboratory of Dr. Eric Vivier |
N/A |
| C57BL/6J R26-LSL-DTA |
Jackson Laboratory |
009669 |
| Oligonucleotides |
|
|
| Synthetic DNA sequences see Table S1
|
|
|
| Recombinant DNA |
|
|
| Cas9 mRNA |
Trilink |
L-7606 |
| MSCV2.2 |
Addgene |
60206 |
| Software and Algorithms |
|
|
| Prism |
GraphPrism software |
Version 7.0 |
| Flowjo |
TreeStar software |
Version 10 |
