Table 4.
Item | Control | LSO | HLS | SEM | P-value |
---|---|---|---|---|---|
Total identified fatty acids methyl esters (mg/mL rumen liquid) | 1.44b | 1.50b | 2.06a | 0.11 | 0.003 |
Saturated fatty acids | |||||
C6:0 | 2.19a | 2.07a | 1.47b | 0.07 | <.0001 |
C8:0 | 1.74a | 1.61ab | 1.29b | 0.09 | 0.022 |
C10:0 | 1.69a | 1.58ab | 1.27b | 0.09 | 0.025 |
C11:0 | 0.87a | 0.83ab | 0.66b | 0.04 | 0.021 |
C12:0 | 2.17 | 2.20 | 1.81 | 0.10 | 0.037 |
C13:0 | 0.94 | 1.02 | 0.97 | 0.04 | 0.399 |
C14:0 | 3.58 | 3.74 | 3.63 | 0.19 | 0.830 |
C15:0 | 2.16 | 2.15 | 1.99 | 0.08 | 0.329 |
C16:0 | 14.80a | 13.65a | 11.96b | 0.28 | <.0001 |
C17:0 | 2.08 | 1.87 | 1.95 | 0.09 | 0.238 |
C18:0 | 17.19 | 18.21 | 19.52 | 0.74 | 0.275 |
C20:0 | 2.34 | 2.36 | 2.12 | 0.06 | 0.521 |
C21:0 | 0.89a | 0.86a | 0.65b | 0.05 | 0.006 |
C22:0 | 2.01a | 1.93ab | 1.62b | 0.09 | 0.016 |
C23:0 | 0.88a | 0.85a | 0.65b | 0.05 | 0.012 |
C24:0 | 2.07a | 1.97a | 1.62b | 0.09 | 0.010 |
Monounsaturated fatty acids | |||||
C14:1 | 1.65 | 1.62 | 1.48 | 0.08 | 0.361 |
C15:1 | 0.69 | 0.74 | 0.66 | 0.03 | 0.235 |
C16:1 | 1.96a | 1.79ab | 1.55b | 0.10 | 0.038 |
C17:1 | 0.97a | 0.90a | 0.68b | 0.05 | 0.008 |
C18:1n9t | 7.23b | 10.69a | 13.13a | 0.60 | 0.0002 |
C18:1n9c | 10.97a | 9.35b | 10.55ab | 0.32 | 0.019 |
C20:1 | 0.95a | 0.94a | 0.73b | 0.05 | 0.018 |
C22:1 | 0.90a | 0.87a | 0.66b | 0.05 | 0.012 |
C24:1 | 1.11a | 0.89ab | 0.66b | 0.11 | 0.053 |
n-6 Polyunsaturated fatty acids | |||||
C18:2n6t | 0.86 | 0.83 | 0.73 | 0.05 | 0.211 |
C18:2n6c | 6.31a | 5.65ab | 4.94b | 0.29 | 0.037 |
C18:3n6 | 0.87 | 0.85 | 0.71 | 0.05 | 0.058 |
C20:2n6 | 0.86a | 0.82ab | 0.64b | 0.05 | 0.017 |
C20:3n6 | 0.82 | 0.80 | 0.68 | 0.04 | 0.093 |
C20:4n6 | 0.90a | 0.86a | 0.67b | 0.04 | 0.006 |
C22:2n6 | 0.88a | 0.84a | 0.62b | 0.05 | 0.006 |
n-3 Polyunsaturated fatty acids | |||||
C18:3n3 | 1.69b | 2.15b | 5.68a | 0.25 | <.0001 |
C20:3n3 | 0.99 | 0.81 | 0.60 | 0.11 | 0.065 |
C20:5n3 | 0.89a | 0.82a | 0.64b | 0.05 | 0.008 |
C22:6n3 | 0.93a | 0.90a | 0.66b | 0.05 | 0.008 |
Sum and ratio1 | |||||
SFA | 57.58a | 56.88ab | 53.31b | 0.83 | 0.016 |
MUFA | 26.42b | 27.79ab | 30.11a | 0.68 | 0.010 |
PUFA | 16.03 | 15.31 | 15.91 | 0.55 | 0.669 |
n-3 PUFA | 4.22b | 4.68b | 5.85a | 0.18 | 0.0004 |
n-6 PUFA | 9.99a | 10.63a | 7.77b | 0.42 | 0.002 |
n-3 LCPUFA | 2.44a | 2.52a | 1.72b | 0.12 | 0.001 |
n-6 LCPUFA | 3.19a | 3.31a | 2.35b | 0.15 | 0.002 |
n-6/n-3 | 2.54a | 2.29a | 1.20b | 0.09 | <.0001 |
P/S | 0.28 | 0.26 | 0.29 | 0.01 | 0.366 |
a–cMeans within the same row followed by the same superscript letters are not significantly different at P < 0.05. The number of observations for each mean value was eight (n = 8).
1SFA saturated fatty acids (6:0 + 8:0 + 10:0 + 11:0 + 12:0 + 13:0 + 14:0 + 15:0 + 16:0 + 17:0 + 18:0 + 20:0 + 21:0 + 22:0 + 23:0 + 24:0), MUFA monounsaturated fatty acids (14:1 + 15:1 + 16:1 + 17:1 + 18:1n9t + 18:1n9c + 20:1 + 22:1 + 24:1), n-6 PUFA n-6 polyunsaturated fatty acids (18:2n6t + 18:2n6c + 18:3n6 + 20:2n-6 + 20:3n6 + 20:4n6 + 22:2n6), n-3PUFA n-3 polyunsaturated fatty acids (18:3n3 + 20:3n3 + 20:5n3 + 22:6n3), PUFA polyunsaturated fatty acids (n-6 PUFA + n-3 PUFA), n-6LCPUFA n-6 long chain polyunsaturated fatty acids (20:2n6 + 20:3n6 + 20:4n6 + 22:2n6), n-3LCPUFA n-3 long chain polyunsaturated fatty acids (20:3n3 + 20:5n3 + 22:6n3); n-6/n-3 = n-6 long chain polyunsaturated fatty acids/n-3 long chain polyunsaturated fatty acids; P/S = polyunsaturated fatty acids/saturated fatty acids.