Table 3.
Primer sequences used for quantitative PCR
| Gene | Primer sequence | Product size, bp | GenBank accession |
|---|---|---|---|
| TNFα | F: CACCACGCTCTTCTGCCTAC | 132 | X57321 |
| R: ACGGGCTTATCTGAGGTTTGAGACG | |||
| IL-1B | F: TGGCCCACACATGCTGAA | 84 | NM_214055 |
| R: CCTTGCACAAAGCTCATGCA | |||
| IL-6 | F: GGCTGTGCAGATTAGTACC | 124 | AF518322 |
| R: CTGTGACTGCAGCTTATCC | |||
| IL-8 | F: AGGACCAGAGCCAGGAA | 172 | NM_213867 |
| R: GTGGAATGCGTATTTATGC | |||
| IL-10 | F: TGGGTTGCCAAGCCTTGT | 61 | L20001 |
| R: GCCTTCGGCATTACGTCTTC | |||
| CLDN1 | F: GATTTACTCCTACGCTGGTGAC | 199 | AJ318102 |
| R: CACAAAGATGGCTATTAGTCCC | |||
| CLDN3 | F: TTGCATCCGAGACCAGTCC | 85 | NM_001160075 |
| R: AGCTGGGGAGGGTGACA | |||
| OCLN | F: AACTCCCGTCAGCAGATCC | 95 | NM_001163647 |
| R: ATCAGTGGAAGTTCCTGAACCA | |||
| ZO-1 | F: CTCTTGGCTTGCTATTCG | 197 | XM_003353439 |
| R: AGTCTTCCCTGCTCTTGC | |||
| TLR4 | F: CAGATAAGCGAGGCCGTCATT | 113 | AB232527 |
| R: TTGCAGCCCACAAAAAGCA | |||
| CD14 | F: CCTCAGACTCCGTAATGTG | 180 | AB267810 |
| R: CCGGGATTGTCAGATAGG | |||
| MYD88 | F: GCTGGAACAGACCAACTAT | 153 | NM_001099923.1 |
| R: TCCTTGCTTTGCAGGTAAT | |||
| CFTR | F: ACTATGGACCCTTCGAGCCT | 123 | NM_001104950 |
| R: CGCATTTGGAACCAGCGTAG | |||
| RPL19 | F: AACTCCCGTCAGCAGATCC | 147 | AF435591 |
| R: AGTACCCTTCCGCTTACCG |
TNFα = tumor necrosis factor alpha; CLDN1 = claudin-1; CLDN3 = claudin-3; OCLN = occludin; ZO-1 = zonula occludens-1; TLR4 = toll-like receptor 4; CD14 = cluster of differentiation 14; MYD88 = myeloid differentiation primary response 88; CFTR = cystic fibrosis transmembrane conductance regulator; RPL19 = ribosomal protein L19.