Figure 1.

Transgenic animals design and tamoxifen treatments. A: The PDGFRα‐CreER mice were crossed with mT/mG reporter mice to produce PDGFRα‐CreER;mT/mG mice. Tamoxifen treatment induces Cre recombinase expression, then the dTomato transgene is deleted and allows the expression of the mEGFP. B: Pregnant mice were orally fed with tamoxifen (2 mg in 100 μL sunflower oil) for five, four, three, or two consecutive days starting at E13.5 and placentas collected at E18.5. C: Tamoxifen was administrated to transgenic mice at E13.5 and E14.5. Placentas were harvested at 18.5. D: A five‐day tamoxifen treatment was administrated to pregnant mice from E7.5 to E11.5; tissues were collected at E12.5. CreER, tamoxifen‐dependent Cre recombinase; E, embryonic day; mEGFP, membrane‐localized enhanced green fluorescent protein; PDGFRα, platelet‐derived growth factor receptor alpha