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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Clin Exp Allergy. 2019 Jan 8;49(5):615–625. doi: 10.1111/cea.13322

Figure 5. Polyclonally stimulated B cells sorted from B cell clusters identified by flowType are capable of producing alpha-gal sIgE in meat allergic subjects.

Figure 5.

(A) Gating strategy for sort purification of B cell subsets grouped into 4 main clusters, with each cluster indicated by a colored gate. (B and C) B cells of each cluster from meat allergic (n = 14) and non-meat allergic control (n = 10) subjects were cultured 8 days and analyzed for alpha-gal sIgE antibody-secreting cells (ASC) by ELISPOT. (B) Representative ELISPOT assay images from 1 allergic and 1 control subject. (C) Frequencies of alpha-gal sIgE ASCs of each B cell cluster after culture expressed as the number of ASCs per million cells. P-values are shown in each panel.