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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Brain Behav Immun. 2019 Jan 23;78:131–142. doi: 10.1016/j.bbi.2019.01.015

Figure 4. RSD in Female Mice Promotes Microglial Restructuring and Monocyte Accumulation in the Brain.

Figure 4.

Adult female C57BL/6 mice were subjected to six cycles of modified repeated social defeat (RSD) for 30 minutes per day using a male DREADD aggressor. Mice were perfused and PFA-fixed 14 h following the final cycle of RSD. Brains were sectioned and Iba-1 was determined (n=6 per group). A) Representative Iba-1 labeling in the basolateral amygdala (BLA) of control and RSD mice. B) Percent area of Iba-1 labeling and C) microglia cell counts per field of vision in the BLA. D) Percent area of Iba-1 labeling in the motor cortex. E) In a separate study, RNA was isolated from a coronal brain slice that included the hippocampus and amygdala 14 h following the last cycle of RSD and IL-1β and TNFα mRNA levels were determined (n=6). In a separate study, mice were subjected to RSD as above and the cell from the brain were Percoll-enriched (n=6). F) Representative bivariate dot plots of CD11b and CD45 labeling in the brain. G) Percentage of microglia (CD11b+/CD45med) and monocytes (CD11b+/CD45hi) in the brain 14 h after RSD.