Table 1.
Primers and Probes. Asterisks (*) indicate reported in this paper, plus signs (+) indicate a locked nucleic acid at the preceding nucleotide position.
| Primer Name | Sequence (5′-3′) | Reference |
|---|---|---|
| JVSF 18S | AGCCTTTCAGCCGTATCTGT | Narayanan et al. (2015) |
| JVSP 18S | /FAM/AGGCC/ZEN/TGCCTTGAGCACT/IABkFQ/ | Narayanan et al. (2015) |
| JVSR 18S | TCGGGAGCGGACGGCATCTTTA | Narayanan et al. (2015) |
| CO1R15 | TGAGCWAYHACAAAYCAHGTATC | (Brant and Loker, 2009a,b) |
| CO1F15 | TTTNTYTCTTTRGATCATAAGC | (Brant and Loker, 2009a,b) |
| SRTP FWD | TGGTTTGGTWTGTGCTATGGG | * |
| SRTP PRB | /FAM/TGAGC + TCA + TACTACACTACC + TAAAC/IABkFQ/ | * |
| SRTP REV | AKTCTTAACATCCAATCCY | * |
| SRTS FWD | ATTATCTAATTACTAATCATGGGATTGCA | * |
| SRTS PRB | /FAM/ACCAAACCC/ZEN/ACCAATCAATACAGGCA/IABkFQ/ | * |
| SRTS REV | ATGCCAAATCATCTAAACCCAAC | * |
| SRTSZ FWD | GTTGTTGGGTTCTGTTAAATTTATAAC | * |
| SRSZ PRB | /FAM/TCTTAGTTC/ZEN/TCGGGTTTCGGTTGTTGTT/IABkFQ/ | * |
| SRSZ REV | AGACGTAAACAAATACGCCCA | * |
| SRAB FWD | GATTCCTTCAGAGATTTATAAATATTTA | * |
| SRAB PRB | /FAM/TACCAAACC/ZEN/CRCCAATRAACACRGGCA/IABkFQ/ | * |
| SRAB REV | ACGAGGTAACGCCAAATC | * |
FAM = fluorescein; IABkFQ = Iowa Black fluorescent quencher.