Table 1.

Peptide sequences of the validated NES motifs or false positives used in the structure-based modeling.

Protein	Class	NES sequence	KD (nM)§	ref.
MVM NS2	1a	77STVDEMTKKFGTLTIHD93	2	31
*super PKI	1a	34NLNELALKLAGLDINK49	4	31
PKI	1a	34NSNELALKLAGLDINK49	34	31
ADAR1	1a	121RGVDCLSSHFQELSIYQ137	69	31
MEK1	1a	28TNLEALQKKLEELELDE44	70	31
Pax	1a	264RELDELMASLSDFKFMA280	700	31
*CPEB4-R	1a	395RMIDILSSELSHMDFTR379	710	31
NPMmutA	1a	278MTDQEAIQDLCLAVEEVSLRK298	790	31
HDAC5	1a	1081EAETVSAMALLSVG1095	1600	31
p73	1a	364NFEILMKLKESLELMELVP382	2000	31
*hRio2-R	1a	405GKIEELAQNFETMEFSR389	2600	31
Stradα	1a	413GIFGLVTNLEELEVD427	10300	31
*FMRP-1b	1b	YLKEVDQLRALERLQID	3000	14
SNUPN	1c	1MEELSQALASSFSVSQDLNS20	12500	31
HPV E7	1c	73HVDIRTLEDLLMGTLGIVC91	34000	31
HIV Rev	2	73LQLPPLERLTLDC85	1180	31
FMRP	2	424LKEVDQLRLERLQID438	2000	31
SMAD4	2	134ERVVSPGIDLSGLTLQ149	4600	31
mDia2	3	1157SVPEVEALLARLRAL1171	1600	31
CDC7	3	456QDLRKLCERLRGMDSSTP473	20000	31
X11L2	4	55SSLQELVQQFEALPGDLV72	1500	31
CPEB4	1a-R	379RTFDMHSLESSLIDIMR395	800	31
hRio2	1a-R	389RSFEMTEFNQALEEIKG405	2800	31
*PKImut1 (I47A)	—	34NSNELALKLAGLDANK49	150000	31
*PKImut2 (L42A/L45A)	—	34NSNELALKAAGADINK49	900000	31
†APC	1a	163AQLQNLTKRIDSLPL174	(−)	33
‡Cyclin D1	1a	281VDLACTPTDVRDVDI295	(−)	—
APRIL	1b	106LEPLKKLECLKSLDL120	(−)	33
‡hTERT	1c	965KAGRNMRRKLFGVLRLKC982	(−)	—
DcpS	1c	136TEKHLQKYLRQDLRL150	(−)	33
Cdk5	2	133LINRNGELKLADFGL147	(−)	33
†FGF1	2	138THYGQKAILFLPLPV152	(−)	33
COMMD1	3	171ILKTLSEVEESISTL185	(−)	20
DEAF1	1a-R	452SWLYLEEMVNSLLNTAQQ469	(−)	13
SGN5	1a-R	221YALEVSYFKSSLDRKLL238	(−)	13
†COMMD1–2	1a-R	164DEVKVNQILKTLSEVEES181	(−)	13
†ELF3	1a-R	111RLVFGPLGDQLHAQLR126	(−)	13

^*Engineered or mutated (underscored residues are the ones inserted or mutated).

^†Do not fit the consensus in Fig. 1 (due to Pro or do not have a bulky residue at Φ3/4 in the class 1a-R).

^‡Unpublished data.

^§(−) means no binding determined by pull-down binding assay.