Fig. 1.

EPR displays epithelial expression and antagonizes TGF-β-induced EMT in mammary gland cells. a Quantitative RT-PCR (qRT-PCR) analysis of EPR in NMuMG cells serum-starved (2% FBS, 16 h) and either treated with TGF-β (10 ng ml⁻¹) for the indicated times or untreated (time 0). b qRT-PCR analysis of EPR in the indicated mouse tissues. c NMuMG cells were fractionated and RNA was prepared from cytoplasm, nucleoplasm, and chromatin and analyzed by qRT-PCR to quantify the indicated RNAs. Rnu1a1 is also known as U1 small nuclear RNA, Gapdh mRNA encodes the glyceraldehyde-3-phosphate dehydrogenase. d qRT-PCR analysis of h.EPR in normal human breast cells isolated from reduction mammoplasty specimens²¹. e qRT-PCR analysis of the indicated transcripts in either mock or EPR-overexpressing (EPR) NMuMG cells serum-starved and either treated with TGF-β (+) for 24 h or untreated (−). f Immunoblot analysis of total cell extracts from either mock or EPR-overexpressing (EPR) NMuMG cells. The indicated antibodies were used. The position of molecular mass markers is indicated on the left. Representative gels are shown. ACTB is also known as Actin Beta. g Phase contrast microscopy of either mock or EPR-overexpressing (EPR) NMuMG cells. Scale bars: 100 μm. h qRT-PCR analysis of the indicated transcripts in NMuMG cells transiently transfected with either control siRNA (siC) or siRNA designed to silence EPR expression (siEPR). The values of qRT-PCR experiments shown are averages (±SEM) of three independent experiments performed in triplicate. Statistical significance: *p < 0.01, **p < 0.001 (Student’s t test)