Table 1.
Influence of Onzin and FCR1 on DNA mutation rate measured by the Lys2::insE-A14 reversion assay.
| Strain | Vector | Treatment | Reversion Rate × 10−5 (confidence intervals) | Fold decrease in mutation ratea | nb |
|---|---|---|---|---|---|
| WT | pFL61-EV | Control medium | 0,14 (0,30 ÷ 0,09) | 1 | 30 |
| pFL61-FCR1 | 0,19 (0,21 ÷ 0,13) | 0,74 | 20 | ||
| pFL61-Onzin | 0,18 (0,20 ÷ 0,16) | 0,74 | 20 | ||
| WT | pFL61-EV | CdSO4 1 μM | 5,49 (6,10 ÷ 5,00) | 1 | 30 |
| pFL61-FCR1 | 1,75 (1,88 ÷ 1,54) | 3,14 | 20 | ||
| pFL61-Onzin | 1,92 (1,99 ÷ 1,70) | 2,85 | 20 | ||
| mlh3 | pFL61-EV | Control medium | 1,55 (1,88 ÷ 1,03) | 1 | 25 |
| pFL61-FCR1 | 1,63 (1,80 ÷ 1,20) | 0,95 | 25 | ||
| pFL61-Onzin | 1,57 (1,68 ÷ 1,04) | 0,99 | 25 | ||
| mlh3 | pFL61-EV | CdSO4 1 μM | 14,61 (15,11 ÷ 14,01) | 1 | 25 |
| pFL61-FCR1 | 2,8 (3,01 ÷ 2,03) | 5,21 | 25 | ||
| pFL61-Onzin | 2,55 (2,97 ÷ 2,12) | 5,72 | 25 |
Reversion to Lys+ phenotype was assessed in the wild-type (WT) and mlh3 yeast mutant strains expressing FCR1, Onzin or the empty vector (EV) on both control medium and cadmium-containing medium. ‘n’ column indicates the number of independent cultures tested from at least two independently constructed strains. Median mutation rates are presented as ×10−5 with 95% confidence intervals. Relative mutation rates are compared with the empty vector of the same genetic background strain. aEach median reversion rate was normalized to the empty vector median rate to calculate the fold decrease; bn = number of independent replicates.