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. 2019 Apr 29;12:26. doi: 10.1186/s12284-019-0288-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 9 — Candidate gene analysis for WLS5. a Location of WLS5 on rice chromosome 5. b Coarse linkage map of WLS5. Markers used for mapping are indicated. Numbers below lines indicate number of recombinants. c Candidate genes for WLS5. d LOC_Os05g04900 structure and sequence variations between wild type and wls5. Black and red arrows indicate mutation site and target knockout position, respectively. e, f LOC_Os05g04900 knockout in ‘Nipponbare’ (NPB). CRISPR/Cas9-induced 18-bp deletion in LOC_Os05g04900 (e). Phenotype of NPB and line #20. Bars = 25 cm (left) and 12.5 cm (right) (f)