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. 2019 Apr 29;9:6622. doi: 10.1038/s41598-019-43054-4

Figure 1.

Figure 1

UM171 effect on expansion of CD34+CD43+ hPSC-derived HPs. (A) Schematic diagram of protocol used for expansion of HPs generated on day 9 H1 hESC differentiation in chemically defined conditions. (B) Representative dot plots show CD34 and CD43 expression following 5 and 7 days of expansion with UM171 or DMSO (control). (C) Histograms show that most of the cells in expansion cultures acquire CD45 expression. Dot plot demonstrates enhancing effect of UM171 on CD201 expression by CD34+ cells. (D) UM171 effect on % and absolute numbers of CD34+CD43+CD45+ HPs in cultures of hESC-derived CD43+ cells expanded for 5 and 7 days. Results are mean ± SEM for 7 independent experiments (Day 5), and 6 independent experiments (Day 7). **p < 0.01, ***p < 0.001 (E) CFC potential of expanded cells. Results are mean ± SEM for 7 independent experiments (Day 5), and 6 independent experiments (Day 7). **p < 0.01, ***p < 0.001. Representative images of colonies from HPs expanded with and without UM171 are shown. Image bar is 790 μM. (F) Cytospin showing morphology of granulocytes generated from UM171 expanded hematopoietic progenitors. Image bar is 50 μM. (G) Phenotype of neutrophils generated from hematopoietic progenitors expanded for 3 days with DMSO or UM171. (H) Phagocytosis of zymosan particles by neutrophils. Plots show histograms for cells incubated at 4 °C (filled gray; nonspecific binding control) and 37 °C (filled green). Percentages of FITC-positive cells at 37 °C minus nonspecific binding control at 4 °C are shown.